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激光显微细胞分离技术
在基础医学研究中涉及越来越多的如某一疾病状态组织中，多种基因或遗传变化，区别发展中的组织细胞群以及疾病状态组织细胞，将有助于了解疾病发生的分子机制。因此，在下一
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Assay for Nucleotide Excision Repair Protein Activity Using Fractionated Cell Extracts and UV-Damage
Mammalian cells remove carcinogenic damage caused to DNA by ultraviolet (UV) lig
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Keratinocyte Transient Transfections
The goal of this chapter is to give the reader a concise and easy-to-follow guid
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Quantitation of Sirolimus Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS)
A multiple reaction monitoring positive ion HPLC method with tandem mass spectro
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Direct Peptide Profiling of Brain Tissue by MALDI-TOF Mass Spectrometry
Direct MALDI-TOF mass spectrometric peptide profiling is increasingly used to an
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细胞生物基本方法：常规组织培养法
常规组织培养法 1 ）初代消化培养法 1. 准备：取各种已消毒的培养用品置于净化台面，紫外线消毒 20 分钟。开始工作前先洗手、 75% 酒精擦拭手至
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Well-Differentiated Human Airway Epithelial Cell Cultures
The airway epithelium occupies a critical environmental interface, protecting th
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Deep Sequencing of Small Chromatin-Associated RNA: Isolation and Library Preparation
Chromatin-associated RNA (caRNA) is a newly identified class of RNA molecules st
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多聚核糖体(polyribosomes)
在蛋白质合成过程中,同一条mRNA分子能够同多个核糖体结合，同时合成若干条蛋白质多肽链，结合在同一条mRNA上的核糖体就称为多聚核糖体(polysome 或po
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Over the past few years, the view that little or no cell turnover or replacement takes place within
TheADP-ribosylation factor proteins (ARFs) are ~20-kDa guanine-nucleotidebinding
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Cultures of Stem Cells of the Central Nervous System
Over the past few years, the view that little or no cell turnover or replacement
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CELL ADHESION
CELL ADHESION Procedure:1) Dissolve/dilute coating substrate in ddH2O at 4 C. A
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EB Differentiation
Aspirate medium from a 6-well plate and wash with PBS Ca++/Mg++ free. Incubate f
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胞内运输(intracellular transport)
真核生物细胞内膜结合细胞器与细胞内环境进行的物质交换。包括细胞核、线粒体、叶绿体、溶酶体、过氧化物酶体、高尔基体和内质网等与细胞内的物质交换。
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支原体检测：DNA萤光染色法
原理利用萤光染剂（bisbenzimide, Hoechst 33258）侦测支原体污染。此染剂会结合到DNA之Adenosine-Thymidine (A-T
