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A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidi
Analytical methods characterizing the immunogenicity of antigens are useful for
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白细胞的吞噬作用
吞噬作用是生物体最古老的，也是最基本的防卫机制之一。对于其要消灭的对象无特异性，在免疫学中称之为非特异性免疫作用。中性粒细胞和单核细胞的吞噬作用很强，嗜酸性粒细
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血细胞的分离方法（中性粒细胞、嗜碱性粒细胞
血细胞由红细胞,白细胞,血小板等组成.实验中常用白细胞来做研究,如炎症渗出,细胞迁移,识别等都涉及到白细胞.白细胞又分为:粒细胞,淋巴细胞,单核细胞等.它们具有
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荧光标记法检测细胞凋亡的操作步骤
相关专题 一、仪器与试剂1. TdT酶(25U/μl);2. 生物素标记的-dUTP(Biotin-dUTP)或地高辛标记的dUTP (Digoxing
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细胞计数和台盼蓝染色
血球计数板每一大方格长为1mm ，宽为1mm ，高为0.1mm ，体积为0.1mm3 ，可容纳的溶液是0.1l ，那么每ml 溶液中所含细胞数即是视野中每一大
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菌体/细胞裂解法汇总
一、反复冻融法： 1、将细胞在-20度以下冰冻，室温融解，反复几次，由于细胞内冰粒形成和剩余细胞液的盐浓度增高引起溶胀，使细胞结构破碎。 2、至少3次以上冻溶。
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抗血清保存的三种方法
相关专题 细胞培养基的配制 细胞培养专题抗血清保存有三种方法：第一种是4℃保存，将抗血清清除菌后，液体状态保存于普通冰箱，可以存放3个月到半年，效价高时，一年之
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Mapping the Regions of RNase P Catalytic RNA That Are Potentially in Close Contact With Its Protein
Ribonuclease P (RNase P) from Escherichia coli is a transfer RNA (tRNA)-processi
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动物细胞转染方法
磷酸钙转染[实验原理]磷酸钙沉淀法是基于磷酸钙-DNA复合物的一种将DNA导入真核细胞的转染方法，磷酸钙被认为有利于促进外源DNA与靶细胞表面的结合。磷酸钙-D
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Large Scale Tubulin Preparation
Large Scale Tubulin Preparation Tubulin is purified from bovine/porcine brain by
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Assays for Human DNase I Activity in Biological Matrices
Pulmozyme� recombinant human deoxyribonuclease I (DNase I) is currently used as
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Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryoni
This chapter introduces the representative method to culture human embryonic ste
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STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination
Inter- and intraspecies cross-contaminations (CCs) of human and animal cells rep
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Cell-Surface ProteinProtein Interaction Analysis with Time-Resolved FRET and Snap-Tag Technologies
F�rster resonance energy transfer (FRET) is a proximity-dependent quantum effect
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Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood
This chapter describes a robust method for the generation of iPS cells from non-
