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Generation of Antibodies That Are Externally Acting Isoform-Specific Inhibitors of Ion Channels
There is demand for isoform-specific ion channel inhibitors as tools to investig
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小鼠白细胞介素21(IL-21)酶联免疫分析(ELISA)
小鼠白细胞介素21(IL-21) 酶联免疫 分析( ELISA )试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定小鼠血清,血浆及
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大鼠白细胞介素6(IL-6)酶联免疫分析
大鼠白细胞介素6 (IL-6 ) 酶联免疫分析试剂盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆,细胞上清及相关液体样本中
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肿瘤细胞培养技术要点
1、取材:材料主要来源于外科手术或活检瘤组织,取材时避免用坏死组织,要挑选瘤细胞集中和活力较好的部位,瘤性转移淋巴结或胸腹水是较好的培养材料。取材后尽快培养,因
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Lentivirus的包装和感染
相关专题 1 转染前一天将293T细胞铺于10cm板,当细胞密度达到80%左右即可进行PEI转染。 2将下列DNA加入一个已装有480ul 1* HBS(
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层粘连蛋白(laminin, LN)
层粘连蛋白主要存在于基膜(basal lamina)结构中,是基膜所特有的非胶原糖蛋白, 相对分子质量为820kDa, 含13-15%的糖,有三个亚单位, 即重
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Simultaneous analysis of DNA content and surface
Simultaneous analysis of DNA content and surface immunophenotype using gentle et
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Immunoflourescence and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free C
This chapter describes the procedures in order to do immunofluorescence (IF) mic
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Isolation of a Membrane-Bound Enzyme, 5-Nucleotidase
Membrane enzymes may be peripheral or integral proteins. The attachment of perip
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Generation and Hepatic Differentiation of Human iPS Cells
A method for the generation of human induced pluripotent stem (iPS) cells was es
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Covalent Modification of G-Proteins by Affinity Labeling
The activation of heterotrimeric G-proteins is tightly regulated by the exchange
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ES and TS cell freezing/thawing
ES and TS cell freezing/thawingNeeded:ES cell freezing medium (2x)2x ES cell fre
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Application of a Fusiogenic Peptide GALA for Intracellular Delivery
To enhance the cytosolic delivery of therapeutic drugs and genes, pH-sensitive a
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大鼠内皮细胞的培养方案
本人总结了下大鼠内皮细胞的培养方案 一,消化法。优点:获得的细胞比其他方法纯。缺点:获得细胞比较少。 常见问题及解决方法:1.消化的时间不好控制时,建议大家分两
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Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold
The study of neuronal connectivity requires the ability to trace axons from the