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Methods for Detecting Mutations in the Human JAK2 Gene
Mutations in the JAK2 gene are prevalent in the human myeloid malignancies, bein
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Cloning by Limiting Dilution of Hybridoma
Author: Nanci DonackiSource: Contributed by Nanci DonackiDate Added: Tue May 14
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胞吐作用(exocytosis)
运输小泡通过与细胞质膜的融合将内容物释放到细胞外基质的过程称为胞吐作用, 膜融合是通过融合蛋白的帮助完成的。在组成型分泌活动中,胞吐作用是自发进行的,但是在调节
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Xeno-Free Culture of Human Pluripotent Stem Cells
Stem cell culture systems that rely on undefined animal-derived components intro
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锌指结构基元(zinc finger motif)
这类蛋白因子往往含有几个相同的指结构, 每一个指结构具有以下的保守序列: Cys-X2-4-Cys-Phe-X5-Leu-X2-His-X3-His。这段保守序
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Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins
One of the most remarkable and unexpected advances in cell biology in the 1990s
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In Vitro Assays for Cobblestone Area-Forming Cells, LTC-IC, and CFU-C
Various assays exist that measure the function of hematopoietic stemcells (HSCs)
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人白细胞介素33(IL-33)酶联免疫分析(ELISA)
人 白细胞介素33(IL-33) 酶联免疫 分析( ELISA )试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相
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Engineering of a 3D Nanostructured Scaffold Made of Functionalized Self-Assembling Peptides and Enca
Three-dimensional (3D) in vitro models of cell culture aim to fill the gap betwe
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Analysis of Suppressor of Cytokine Signalling (SOCS) Gene Expression by Real-Time Quantitative PCR
The Suppressor of Cytokine Signalling (SOCS) proteins are a family of negative r
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细胞培养常见问题解析
相关专题1、如何选用培养基?培养某一类型细胞没有固定的培养条件。在MEM中培养的细胞,很可能在DMEM或M199中同样很容易生长。总之,首选MEM做粘附细胞培养
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Freezing cells in liquid nitrogen
Take off Media Trypsinate with 1ml x2 Dulbecco A trypsin Add 7ml Media Pipette u
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Analysis of Pyroptosis in Bacterial Infection
Eukaryotic cells undergo death by several different mechanisms: apoptosis, a cel
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DNA导入哺乳动物细胞常见的方法
名称 机制 转染效率 用途 优缺点 影响因素 磷酸钙转染法 内吞作用 20%细胞被转染 瞬时表达 1、简单有效 2、适用于贴壁、非贴壁细胞 3、常作首选方法
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Chlamydomonas reinhardtii: The Model of Choice to Study Mitochondria From Unicellular Photosynthetic
Chlamydomonas reinhardtii is a model organism to study photosynthesis, cellular