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Xenopus Oocyte Wound Healing as a Model System for Analysis of Microtubule-Actin Interactions
Microtubule-actin interactions are fundamental to many cellular processes such a
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High Resolution Fluorescent In Situ Hybridization in Drosophila
Tissue-specific gene expression is a major determinant in the elaboration of cel
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An Improved Method for Generating and Identifying Human Induced Pluripotent Stem Cells
This chapter describes the methods we use to derive and characterize human induc
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Cultures of Human Adipose Precursor Cells
Recently, there has been rapidly growing interest in cell culture models that al
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Primary Mouse Keratinocyte Culture
Mouse epidermal keratinocytes have traditionally been difficult to grow in vitro
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Gene Expression in Mouse Oocytes by RNA-Seq
Second-generation sequencers such as the Illumina GAIIx make possible the study
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Localization of mRNA in Vertebrate Axonal Compartments by In Situ Hybridization
The conclusive demonstration of RNA in vertebrate axons by in situ hybridization
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The In Vivo Evaluation of Active-Site TOR Inhibitors in Models of BCR-ABL+ Leukemia
Preclinical evaluation of candidate anticancer compounds requires appropriate an
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Analysis of Small Hsp Phosphorylation
Small heat-shock proteins (sHsps) are involved in diverse biological phenomena a
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Electrical Impedance Spectroscopy for Rapid and Noninvasive Analysis of Skin Electroporation
Transient disruption of skin’s barrier properties using high-voltage pulses invo
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Preparation of Segmented and Polarity Marked Microtubules
Segmented and polarity-marked microtubules are very useful for many different ty
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Chemotaxis in Neutrophil-Like HL-60 Cells
Asymmetric localization of intracellular proteins and signals directs movement d
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Immunolocalization in the Epidermis
Immunohistochemistry is an efficient means of localizing specific proteins to th
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In Vivo Analysis of Ribonucleoprotein Complexes Using Nucleotide Analog Interference Mapping
Multicomponent RNA-protein complexes are essential for eukaryotic gene expressio
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Noncovalent Protein Interaction with Poly(ADP-Ribose)
Compared to most common posttranslational modifications of proteins, a peculiari
