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Methods of Sperm DNA Extraction for Genetic and Epigenetic Studies
High quality DNA extractions developed for mammalian somatic cells are ineffecti
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Analysis of the Rit Subfamily GTPase-Mediated Signaling and Neuronal Differentiation and Survival
The Rit subfamily of GTPases is a founding branch within the Ras family of small
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Protoplast Transformation of Filamentous Fungi
The protoplast method for the transformation of filamentous fungi is described i
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Purification of Peripheral Blood Natural Killer Cells
The ability to perform biological studies on natural killer (NK) cells requires
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Detection of Microsatellite Instability in Cancers by Means of Nongel-Sieving Capillary Electrophore
Microsatellite instability has been shown to be relevant to various human diseas
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ES Cell Culture and Manipulation
MediaHigh glucose DMEM (-pyruvate, -glutamine)20% Heat-inactivated Fetal calf se
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Peptide Nanofiber Scaffolds for Multipotent Stromal Cell Culturing
Self-assembled peptide nanofibers are versatile materials providing suitable pla
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Use of Polyamine Metabolites as Markers for Stroke and Renal Failure
Acrolein and H2 O2 are among the metabolic products of spermine and spermidine,
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Plastic dishes for growing cells
There are two kinds of dishes used to grow tissue culture cells.Those that are d
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Simultaneous Fluorescence Immunostaining and FISH
A visualization method that combines simultaneous fluorescence immunostaining an
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The Basis of Electrochemotherapy
Antitumor electrochemotherapy is a treatment of solid tumors which combines a cy
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Isolation and Functional Analysis of Mitochondria From the Nematode Caenorhabditis elegans
Mitochondria are essential organelles with central roles in diverse cellular pro
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ATP Production in Isolated Mitochondria of Procyclic Trypanosoma brucei
This chapter describes a luciferase-based protocol to measure adenosine triphosp
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Culture Systems: Fluid Dynamic Embryo Culture Systems (Microfluidics)
The tubal/uterine lumen is a dynamic environment in which oocytes, eggs, and ear
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Liposomes for Specific Depletion of Macrophages from Organs and Tissues
A liposome mediated macrophage “suicide” approach has been developed, based on t