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Assay of Deoxyhypusine Synthase Activity
Deoxyhypusine synthase catalyzes an unusual protein modification reaction. A por
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核孔运输(transport through nuclear pore)
胞质溶胶中合成的蛋白质穿过细胞核内外膜形成的核孔进入细胞核。核孔运输又称为门运输，核孔是如同一扇可开启的大门，而且是具有选择性的门，能够主动运输特殊的生物大分子
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Electrotransfection with Intracellular Buffer
Introduction of foreign molecules, such as DNA, RNA, or proteins, into living ce
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Multidimensional Proteomic Analysis of Proteolytic Pathways Involved in Cell Cycle Control
Many cell cycle transitions are controlled by ubiquitin-mediated proteolysis of
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Effects of Rho Family GTPases on Cell-Cell Adhesion
Cell-cell adhesion is dynamically rearranged in various cellular processes, incl
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中心体
中心体存在于低等植物细胞（如衣藻、团藻等藻类植物）和动物细胞中。中心体不具备膜结构，是由蛋白质组成的。每个中心体是由两个互相垂直的短棒状的中心粒排列而成，每个中
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Modulation of Potassium Ion Channel Proteins Utilising Antibodies
The application of antibodies to living cells has the potential to modulate the
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Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation
Ubiquitination and protein degradation regulate cell cycle progression in all eu
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Purification of Murine Monoclonal Antibodies
Hybridoma technology has made possible the production of highly specific, homoge
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Characterization of Enzyme Activity, Protein Content, and Thiol Groups in Immobilized Enzymes
Immobilization is designed to restrict the freedom of movement of an enzyme and,
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Oocyte Isolation and Enucleation
Xenopus laevis oocytes are popular cells in experimental biology. Fully grown oo
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Studying Cytoskeletal Dynamics in Living Cells Using Green Fluorescent Protein
The cytoskeleton is a dynamic structure comprised of at least three distinct cel
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Determination of Gene Expression Patterns by Whole-Mount In Situ Hybridization
Whole-mount in situ hybridization (WISH) is a reliable and specific method to st
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Detection of Apoptosis in the Central Nervous System
Apoptosis occurs in the nervous system during normal development, but can also b
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Serum-Resistant Lipoplexes in the Presence of Asialofetuin
Vectors proposed for gene delivery generally fall into two categories: viral and
