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Isolation of Song-Regulated Genes in the Brain of Songbirds
We have applied the differential display (DD) technique (1 ) to isolate genes wh
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Chick Embryos: Incubation and Isolation
The following methods comprise a brief description of chick egg incubation and e
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Identification of Neural Stem Cells in the Drosophila Larval Brain
The balance between self-renewal and differentiation must be tightly regulated i
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转细胞运输(transcellular transport)
这种运输不仅仅是物质进出细胞,而是从细胞的一侧进入,从另一侧出去,实际上是穿越细胞的运输。在多细胞生物中,整个细胞层作为半渗透性的障碍,而不仅仅是细胞质膜。如植
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Immunogold Labeling for Electron Microscopy
Colloidal gold immunocytochemistry was first introduced by Faulk and Taylor (1 )
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Lentiviral Gene Transduction of Mouse and Human Stem Cells
This chapter describes the methods we use to transduce mouse and human hematopoi
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Application of Functional Blocking Antibodies: N-Cadherin and Chick Embryonic Limb Development
The current trend in developmental biology research is to identify candidate fun
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The Mammalian Cell Cycle: An Overview
In recent years, we have witnessed major advances in our understanding of the ma
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Ultrastructural Analysis of Myoblast Fusion in Drosophila
Myoblast fusion in Drosophila has become a powerful genetic system with which to
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Determination of Fatty Acid Profiles and TAGs in Vegetable Oils by MALDI-TOF/MS Fingerprinting
Vegetable oils are complex mixtures containing a wide range of major compounds.
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Manual Methods for Sperm Motility Assessment
Progressive motility is a vital functional characteristic of ejaculated human sp
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Identification and Characterization of Programmed Cell Death Markers in Bacterial Models
In eukaryotic organisms facing terminal stress, activation of genetically encode
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Preparation of Substrata for In Vitro Culture of Neurons
Cell adhesion, cell contact, and intercellular interactions are critical to neur
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Cloning Differentially Expressed Genes by Using Differential Display and Subtractive Hybridization
Differential gene expression occurs in all phases of life, including development
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Genomic Amplification with Transcript Sequencing (GAWTS)
Genomic amplification with transcript sequencing (GAWTS) ( 1 , 2 ) is a generall