-
Protein-Thiol Oxidation, From Single Proteins to Proteome-Wide Analyses
Protein-thiol oxidation subserves multiple biological functions, from enzymatic
-
Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model Systems
The inflammatory response is a critical component of host defense. An important
-
Stabilization of Enzymes by Multipoint Covalent Immobilization on Supports Activated with Glyoxyl Gr
Stabilization of enzymes via immobilization techniques is a valuable approach in
-
Treatment of Multiple Spontaneous Breast Tumors in Mice Using Electrochemotherapy
Electrochemotherapy has been developed on the basis of in vitro data showing the
-
Isolation and Culture of Rabbit Embryonic Stem Cells
Mammalian stem cells are invaluable research resources for the study of cell and
-
Transgenic Systems in Nuclear Reprogramming
Transgenic reporters have proved to be invaluable in the study of nuclear reprog
-
Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High-Densit
Reconstituted high-density lipoprotein particles (rHDL) are powerful platforms u
-
Genetic Manipulation of Keratinocyte Stem Cells with Lentiviral Vectors
The epidermis of the skin and its appendages, such as the hair follicles, are fo
-
Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aort
We describe a method to derive cell lines and clones from cells of the murine mi
-
Studying Nuclear Reprogramming with Cell Hybrids
Every cell in a mammal, other than odd exceptions (e.g. mature erythrocytes or l
-
The Identification of Subcellular Fractions from Mammalian Cells
The chapters in the two volumes of Biomembrane Protocols are broadly devoted to
-
Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle
The method described in the following chapter utilizes a double thymidine block
-
Methods for Studying Checkpoint Kinases Chk1
Attempts to passage through mitosis with unrepaired DNA damage or incompletely r
-
Prespliceosome and Spliceosome Isolation and Analysis
Spliceosomes are multicomponent enzymes that remove introns from premessenger RN
-
Long-Term B-Lymphoid Cultures from Murine Bone Marrow Establishment and Cloning by Using Stromal Cel
Nearly all hematopoietic cells in mammals derive from precursors that undergo mu
