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High-Throughput FACS Method for Directed Evolution of Substrate Specificity
Directed evolution is a powerful technology for the isolation of enzyme variants
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Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemi
Poly (ADP-ribosyl)ation of proteins is a posttranslational modification mediated
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Fate of Desmosomal Proteins in Apoptotic Epidermal Cells
In epidermal cells desmosomes represent major sites of basolateral cell-cell con
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ISH Probes Derived from BACs, Including Microwave Treatment for Better FISH Results
Besides the well-known applications of bacterial artificial chromosomes (BACs) i
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Detection of Ubiquitination in 2DE
Ubiquitination involves the tagging of proteins with one (mono-) or more (poly-)
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Redox-Sensitive Green Fluorescent Protein: Probes for Dynamic Intracellular Redox Responses. A Revie
The quantification of transient redox events within subcellular compartments, su
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Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection
Epithelial tissues exhibit optimal conditions for studying cellular differentiat
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In Vivo SELEX in Vertebrate Cells
Iterative selection strategies have been widely used to enrich specific RNA mole
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Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedur
Carbohydrates represent a fundamental building unit of living organisms. Many co
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In Vitro Maturation (IVM) of Porcine Oocytes
Oocyte maturation is a critical component of in vitro embryo production. If not
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Nuclear Transfer in Cattle
For decades, a dream of animal breeders has been to clone (make exact genetic co
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Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins
S100 proteins are the largest subgroup of Ca2+ binding proteins with the EF-hand
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推荐一种简单实用的细胞冻存方法
我们实验室一直用一种特简单但非常安全的细胞冻存方法，与大家共享： 冻存液：一般用10%DMSO(推荐用sigema原装的，质量好不用预消毒)+10％血清的完全培
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Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes
The carbohydrate residues of glycosphingolipids were implicated in many biologic
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In vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation, Motility, Tub
This chapter covers the breakdown of the process of angiogenesis into simple ass
