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Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells
The advancement of Drosophila germline stem cell research accompanies the develo
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Site-Directed Mutagenesis Using a Rapid PCR-Based Method
Site-directed mutagenesis is one of the fundamental tools available to probe the
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Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary
Understanding how cell adhesion to the extracellular matrix controls mammalian d
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Tips and Tricks for mFISH
Genetic analysis has been vastly improved by the introduction of the fluorescenc
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Purification and Characterization of Poly(ADP-Ribosyl)ated DNA Replication/Repair Complexes
PARP-1, the best studied isoform and most abundantly expressed member of the PAR
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FISH Targeting of Chromosomes and Subchromosomal Regions in Yeast
Yeasts represent powerful genetic model organisms, due in part to their tiny gen
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High-Resolution NMR of DNA and Drug-DNA Interactions
The advantage of NMR over most other spectroscopic techniques lies in the abilit
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Derivation and Characterization of Gut-Like Structures From Embryonic Stem Cells
Embryonic stem (ES) cells have a pluripotent ability to differentiate into a var
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Percent Labeled Mitosis Curve Analysis
The measurement of percent labeled mitoses (PLM, or alternatively, FLM, for frac
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Electroporation-Mediated Gene Transfer into Hepatocytes
The study of gene expression regulation relies on the introduction of foreign DN
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Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids
The first measurement of the transmembrane diffusion of phospholipids in membran
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细胞生物基本方法：神经组织细胞培养
神经组织细胞培养1． 获取脑组织后，先仔细剥除脑膜和血管等纤维成分，置入 Hanks 液中漂洗 1 ～ 2 次后，置于 30 ～ 50 倍的 Hanks 液中，
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Antisense-Mediated Inhibition of Protein Synthesis: Rational Drug Design, Pharmacokinetics, Intracer
There is hardly any class of drugs for which the term “ratronal drug design” is
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Establishment and Maintenance of In Vitro Cultures of Human Retinal Pigment Epithelium
The retinal pigment epithelium (RPE) is a layer of multipotential cells of neura
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Protein Extraction for 2DE
Our protein extraction protocol for two-dimensional gel electrophoresis (2DE) wa
