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单链断裂重组模型(model of single-strand breaks recombination)
该模型认为:同源染色体在联会期间,一对同源染色体各有一条单链DNA断裂,然后要发生重接,不过在重接的过程中发生了错误,不是原来断开的染色体重新连接起来而是交换连
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Gel Electrophoresis-Based Proteomics of Senescent Tissues
Cellular aging is a fundamental biological process, and mass spectrometry-based
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RAP-PCR Using RNA from Tissue Microdissection
Cell culture provides powerful models for studying cell biology, but many pathol
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Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1)
From Chapter 1 on nucleases, we know that the term DNase refers to an enzyme tha
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Measurement of Activities of Cyclobutane-Pyrimidine-Dimer and (6-4)-Photoproduct Photolyases
The adverse biological consequences of unrepaired toxic UV-B-induced photoproduc
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体外细胞培养对细胞培养基几点要求
相关专题体外培养的细胞直接生活在培养基中,因此培养基应能满足细胞对营养成分、促生长因子、激素、渗透压、pH等诸多方面的要求。营养成分1. 氨基酸:所有细胞都需要
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EB病毒诱导淋巴细胞永生化实验方法
实验步骤: 1. EB病毒液的制备 复苏B95.8细胞株,使用培养基为10% FCS RPMI-1640 逐步添加培养基至细胞长至对数生长期
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Lipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for th
The traditional view of the plasma membrane as a uniform cellular envelope forme
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Ultrasound-Responsive Liposomes
Ultrasound-responsive liposomes are drug-loaded liposomes that contain a small a
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Slug Phototaxis, Thermotaxis, and Spontaneous Turning Behavior
Dictyostelium slug phototaxis and thermotaxis are readily assayed phenotypes tha
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In Situ Localization of Apoptosis Using TUNEL
Apoptosis is an important process both in normal biology and in various patholog
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Quantitative Analysis of miRNA Expression in Epithelial Cells and Tissues
Reliable detection of the microRNA (miRNA) precursor and mature form expression
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Cell-Free Fetal DNA Plasma Extraction and Real-Time Polymerase Chain Reaction Quantification
Isolation, quantification, and genetic analysis of circulating plasma DNA have c
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Clinical Trials for Solid Tumors Using Electrochemotherapy
Chemotherapy is a standard treatment for a wide variety of cancers. However, res
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Neurotransmitter Receptor Analysis in Transgenic Mouse Models
One of the characteristic findings in human Huntington’s disease (HD) is the alt