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Fluorescence In Situ Hybridization Assessment of c-myc Gene Amplification in Breast Tumor Tissues
This chapter details methods used for analysis of DNA copy-number changes in bre
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Apoptosis: A Technical Overview
Physiological or programmed cell death plays an important role during a variety
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Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH)
Differential gene expression represents the entry point for comprehending comple
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Determination of the Catalytic Activities of mTOR and Other Members of the Phosphoinositide-3-Kinase
Members of the phosphoinositide-3-kinase-related kinase (PIKK) family, which inc
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Signal Transduction: A Technical Overview
Delineation of downstream signal transduction pathways is important for determin
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Real-Time RT-PCR (Taqman) of Tumor mRNA to Predict Sensitivity of Specimens to 5-Fluorouracil
5-Fluorouracil (5-FU) is still a key drug in the treatment of various kinds of a
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Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approach
It has been shown that antisense oligodeoxynucleotide (ODN) treatments provide a
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Identification and Characterization of Hematopoietic Stem and Progenitor Cell Populations in Mouse B
The study of key mechanisms and molecules involved in the regulation of hematopo
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Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylate
The major mechanism of imatinib resistance for patients with chronic myeloid leu
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RNAi Technology to Block the Expression of Molecules Relevant to Metastasis: The Cell Adhesion Molec
Specific gene silencing using small hairpin RNA (shRNA) constructs offer researc
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Lymphatic Invasion as a Prognostic Biomarker in Primary Cutaneous Melanoma
Melanoma has a propensity for lymph node metastasis. However, the incidence of l
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Kinetworks Protein Kinase Multiblot Analysis
The proteomics analysis of protein kinases and other cell-signaling proteins in
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Organizational Issues in Providing High-Quality Human Tissues and Clinical Information for the Suppo
Superior-quality human tissues are required to support many types of biomedical
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Using a Quartz Crystal Microbalance Biosensor for the Study of Metastasis Markers on Intact Cells
The use of biosensors has become a standard method to characterize biomolecular
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The Hollow Fiber Assay
The hollow fiber assay is a unique in vivo model that allows simultaneous evalua
