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Enzymatic Isolation and Serum-Free Culture of Human Renal Cells: Retaining Properties of Proximal Tu
Procedures for the culture of the human renal proximal tubule (HPT) cellutilizin
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Examining Planar Cell Polarity in the Mammalian Cochlea
The mammalian cochlea offers a unique opportunity to study the effects of planar
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Characterization of Cross-Linked Actin Filament Gels and Bundles Using Birefringence and Polarized L
Fundamental processes in the life of Dictyostelium , such as locomotion, endocyt
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Assay of Deoxyhypusine Hydroxylase Activity
The eukaryotic translation initiation factor 5A (eIF5A) is the only cellular pro
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Intravital Two-Photon Imaging of Adoptively Transferred B Lymphocytes in Inguinal Lymph Nodes
Intravital two-photon imaging allows the observation of immune cells in intact o
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Integration of Protein Kinases into Transcription Complexes: Identifying Components of Immobilised I
Regulation of gene expression is essential for coordinated cell growth and devel
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The Use of Electrophoretic Mobility Shift Assays to Study DNA Repair
DNA repair pathways must include proteins that recognize and bind to damaged DNA
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Determination of the Activity of Rho-Like GTPases in Cells
Rho-like GTPases, including RhoA, Rac1 and Cdc42, have been implicated in the co
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Methods to Assess Tissue Permeability
An essential requirement for adequate organ performance is the formation of perm
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自体组装(self assembly)
生物大分子借助本身的力量自行装配成高级结构，现代的概念应理解为不需要模板和酶系的催化, 以别于模板组装和酶效应组装。其实，这种组装也需要一种称为分子伴侣的蛋白介
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核糖体
核糖体不是由生物膜构成的，它是由蛋白质和RNA 构成的复合体。由大小两个亚基组成。核糖体是蛋白质合成的场所。附着在内质网上的核糖体合成的蛋白质主要有两类：一类是
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Nucleotide Metabolism and Cell-Cell Interactions
Interactions between the vascular endothelium and polymorphonuclear leukocytes (
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FISH on Sperms, Spermatocytes and Oocytes
It is well known that chromosome in situ hybridization allows the unequivocal id
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细胞转染方法
物理方法 电穿孔(electroporition) 显微注射(microinjection) 化学方法 磷酸钙转染(calcium phosphate tran
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Tissue Culture Media
Tissue Culture MediaWe use two different kinds of media. Most cells are grown in
