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Immobilization of Enzymes and Cells: Some Practical Considerations
The technology for immobilization of cells and enzymes evolved steadily for the
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Structural Analysis of Protein-RNA Interactions With Mass Spectrometry
We present a high-resolution mass spectrometric footprinting approach enabling t
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关于动物血清的常见问题及处理方法
最近在培养细胞中使用胎牛血清,由于发现有沉淀,不知是否变质,在查阅相关文献也没有得到确切的答案.因此不敢再用原来的血清,导致浪费.在这提醒大家做细胞培养时,要注
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Chemical Assays for Proteins
The choice of a protein assay for membranes and membrane proteins is dependent o
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Generation of Human-Induced Pluripotent Stem Cells (hiPSCs) Using Episomal Vectors on Defined Essent
Human-induced pluripotent stem cells (iPSCs) are an important potential source o
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Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a
Colorectal cancer, originating in tissues of epithelial origin, is one of the mo
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Introduction to Nucleocytoplasmic Transport: Molecules and Mechanisms
Nucleocytoplasmic transport, the exchange of matter between nucleus and cytoplas
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Analysis of Biomarkers of Caloric Restriction in Aging Cells
Caloric restriction (CR) has been extensively documented for its profound role i
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常用封闭剂选择的窍门
相关专题 生物实验中的封闭剂杂交膜上有很多非特异性的蛋白质结合位点,为防止这些位点与抗体结合引起非特异的染色和背景,一般用惰性蛋白质或非离子去污剂封闭膜上的未结
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Selective Extraction of Fragmented DNA from Apoptotic Cells for Analysis by Gel Electrophoresis and
A characteristic feature of apoptosis is activation of an endonuclease(s), which
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Identification of Mutated p53 in Cancers by Nongel-Sieving Capillary Electrophoretic SSCP Analysis
The tumor suppressor gene, p53, lies on chromosome 17p, and has been examined in
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Osteogenic Differentiation of Embryonic Stem Cells in 2D and 3D Culture
Osteoblasts are the cells that contribute to the formation and function of bone
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矽肺(silicosis)
空气中的矽(SiO2 )被吸入肺后,被肺部的吞噬细胞所吞噬,由于吞入的二氧化硅颗粒不能被消化,并在颗粒的表面形成硅酸。硅酸的羧基和溶酶体膜的受体分子形成氢键,使
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Activation of the Mitogen-Activated Protein Kinase Pathway by TGF
The mitogen-activated protein kinase (MAPK) superfamily includes three major sub
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Detection of the Senescence-Associated Secretory Phenotype (SASP)
Cellular senescence suppresses cancer by eliminating potentially oncogenic cells