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Ultracentrifugation Technique for Measuring the Binding of Peptides and Proteins to Sucrose-Loaded P
The binding of extrinsic proteins to membranes is important for their biological
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Mass Measurement of Key Phosphoinositide Cycle Intermediates
The phosphoinositide cycle is now established as a major pathway by which a vari
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Basic Protocols to Investigate hMSC Behavior onto Electrospun Fibers
Human mesenchymal stem cells (hMSC) currently represent a major cell resource in
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Isolation of Sea Urchin Sperm Plasma Membranes
Sea urchin spermatozoa provide an excellent model system for studying the signal
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Human Thyroid Epithelial Cells
The thyroid gland contains two populations of epithelial cells, of quite differe
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A High-Throughput Sequencing-Based Methodology to Identify All Uncapped and Cleaved RNA Molecules in
How eukaryotic organisms regulate mRNA levels is a fundamental question in biolo
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Flow Cytometry: Immunofluorescence Staining of Activated and Rest
Flow Cytometry: Immunofluorescence Staining of Activated and Resting Human Plate
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Using TUNEL in Combination with an Active Caspase-3 Immunoassay to Identify Cells Undergoing Apoptos
The term apoptosis was coined in a now classic paper by Kerr et al. in 1972 (1 )
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Isolation of Human Blood DC Subtypes
Human blood dendritic cells (DCs) are a rare, heterogeneous cell population that
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Methods for the Analysis of the Sperm Proteome
Proteomics is the study of the proteins of cells or tissues. Sperm proteomics ai
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Pig Neural Cells Derived from Foetal Mesencephalon as Cell Source for Intracerebral Xenotransplantat
Intracerebral cell transplantation offers the possibility of replacing lost neur
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Freezing Cells
1) Keep prepared solutions on ice. 2) Determine total cell count of cells to be
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Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches
Although embryonic stem cells (ESCs) have been established from mice since 1981,
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The Chick Embryo as a Model System for Analyzing Mechanisms of Development
The chick embryo provides an excellent model system for studying the development
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Kinetic Analysis of the Inhibition of Matrix Metalloproteinases by Tissue Inhibitor of Metalloprotei
The kinetic analysis of the inhibition of matrix metalloproteinases by tissue in