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Construction and Characterization of Minibodies for Imaging and Therapy of Colorectal Carcinomas
Engineering of antibodies specific for tumor-associated antigens provides great
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软琼脂克隆形成实验
原理:细胞接种存活率只表示接种细胞后贴壁的细胞数,但贴壁后的细胞不一定每个都能增殖和形成克隆。而形成克隆的细胞必为贴壁和有增殖活力的细胞。克隆形成率反映细胞群体
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真核生物翻译的调控
原核生物基因表达的调控主要在转录水平上进行,而真核生物由于RNA较为稳定,所以除了存在转录水平的调控以外,在翻译水平上也进行各种形式的调控。在蛋白质生物合成的起
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d-Glucaric Acid as a Prospective Tumor Marker
d -Glucaric acid (GA) 1s a natural, apparently nontoxic compound produced in sma
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Diet, Epigenetics, and Cancer
Cancer encompasses a highly heterogeneous group of diseases. It has been thought
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Interaction of p53 with Cellular Proteins
Cellular proteins that interact with p53 play a major role in both positive and
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有限稀释法
有限稀释法的程序①制备饲养细胞悬液②转染了重组质粒后的细胞计数,并调细胞数在1~5103/ml③取130个细胞放入6.5ml含饲养细胞完全培养液,即20个细胞/
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Enhanced Permeability and Retention (EPR) Effect for Anticancer Nanomedicine Drug Targeting
Effective cancer therapy remains one of the most challenging tasks to the scient
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Multicolor Spectral Karyotyping in Multiple Myeloma
Multiple myeloma is a plasma cell disorder characterized at the cytogenetic leve
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luciferase检测的标准品问题
荧光素酶作为一种报告基因要在检测时比较其相对发光强度值,就要在转染时引入另外一个报告基因进行转染条件、效率的标准化归一。Promega公司在这方面有双荧光素酶检
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吉凯基因分享:结直肠癌研究体内外模型综述
引言:上海吉凯基因化学技术有限公司成立于 2002 年,拥有国内最大的慢病毒文库,同时也是国内最大的疾病关键基因科研服务供应商,在疾病研究领域的实验积累已逾 1
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MicroRNA Biogenesis and Cancer
MicroRNAs are short non-coding RNA molecules that are involved in diverse physio
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Southern Blotting of IgH Rearrangements in B-Cell Disorders
Southern blotting is a method whereby DNA fragments in the gel are denatured by
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Human Solid Tumors: Cytogenetic Techniques
The field of cytogenetics has had a great impact on many aspects of medical and
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细胞组分的分析方法
一.基本原理核酸分子杂交技术是目前分子生生物学、细胞生物学和生物化学研究中应用最广泛的技术之一,是定性、定量和定位检测两条来源不同的聚核苷酸链上碱基顺序同源性的