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三联管(triplet)
见于中心粒(centrioles)和基体(basal bodies),由A、B、C三个单管组成,A管由13根原纤维组成,B管和C管都是10根原纤维,所以一个三联
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Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut
Stem cell-mediated tissue repair is a promising approach in regenerative medicin
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Principles for Derivation of Human Embryonic Stem Cells
This chapter describes the principles for derivation and maintenance of human em
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Protocols for Studying Antizyme Expression and Function
Antizyme (AZ) is a key molecule in feedback regulation of cellular polyamines. I
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The efficient generation of mesenchymal cells such as adipocytes, osteoblasts, and chondrocytes from
Localization of messenger RNA (mRNA) is a process used by eukaryotes to control
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Generation of Osteoblasts and Chondrocytes From Embryonic Stem Cells
The efficient generation of mesenchymal cells such as adipocytes, osteoblasts, a
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Analysis of Epithelial Cell Shedding and Gaps in the Intestinal Epithelium
The intestinal barrier is formed by a monolayer of columnar epithelial cells. Th
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In Vitro Human T Cell Development Directed by NotchLigand Interactions
Traditionally, the study of human T cell development has relied on the availabil
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干细胞(stem cells, SC)
一类具有自我复制能力(self-renewing)的多潜能细胞,在一定条件下,它可以分化成多种功能细胞。干细胞有两种分类方法,一是根据干细胞所处的发育阶段分为胚
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Detecting Expression Patterns of Wnt Pathway Components in Nematostella vectensis Embryos
The anthozoan cnidarian Nematostella vectensis has emerged as a key model system
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巨噬细胞培养方法与步骤
一、巨噬细胞培养设施设备与器皿1、巨噬细胞培养设施设备(1)超净工作台:也称净化工作台,分为侧流式、直流式和外流式三大类。(2)无菌操作间:一般由衣间,缓冲间和
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巨噬细胞分离与纯化步骤
一、细胞分离1、取6周左右的小鼠(或600克左右的豚鼠,或3公斤左右的家兔),剃去腹部的毛并消毒。腹腔注射1ml(豚鼠20ml,家兔200ml)无菌的液体石蜡或
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巨噬细胞流式检测方法
巨噬细胞是先天免疫系统的一种特殊的、长寿命的吞噬细胞。巨噬细胞是三种吞噬细胞类型之一,还有粒细胞(中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞)和树突状细胞(DCs)
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什么是亚硫酸氢钠测序?
亚硫酸氢钠测序法(BSP)是基于亚硫酸氢盐能使DNA中未发生甲基化的胞嘧啶脱氨基转变成尿嘧啶,而甲基化的胞嘧啶保持不变,行PCR扩增所需片段,则尿嘧啶全部转
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亚硫酸氢钠测序(BSP)实验步骤
1、引物设计在GenBank上找到待检基因启动子区的原始序列,输入到“MethPrimer”软件中,输入启动子序列,选择“Pick primers for bi