-
Neural Differentiation of Human ES and iPS Cells in Three-Dimensional Collagen and Martigel Gels
In this chapter, we describe an effective and reproducible protocol for neural d
-
High-Throughput Screening of Microtubule-Interacting Drugs
Drugs that affect microtubule dynamics are among the most effective anticancer a
-
Screen for Oxidases by Detection of Hydrogen Peroxide with Horseradish Peroxidase
Oxidases remove hydrogen from substrates, transferring it to molecular oxygen to
-
流式细胞仪在睡眠性阵发性血红蛋白尿的诊断中的应用
一 . 睡眠性阵发性血红蛋白尿 PNH睡眠性阵发性血红蛋白尿（ PNH ）是一种获得性造血干细胞异常，临床主要表现为骨髓丧失造血功能、血栓、慢性溶血性贫血急性发
-
Counting Chromosomes in Intact Eggs
Chromosomal spreads are an established method to assess ploidy in different cell
-
Viral Transformation of Epithelial Cells
Approximately 18% of human cancers have a viral etiology and the majority of the
-
The MAP Kinase Signaling Cascades: A System of Hundreds of Components Regulates a Diverse Array of P
Sequential activation of kinases within the mitogen-activated protein (MAP) kina
-
Genome-Wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq
Chromatin immunoprecipitation (ChIP) is used to evaluate the interaction of prot
-
Determination of Proteasomal Activities
The proteasome is an important component of the intracellular system for the tur
-
Sequencing-Based Typing of HLA
Human leukocyte antigen (HLA) typing is largely performed by use of PCR-based te
-
Tumor Growth Fraction Estimation, Perturbation, and Prognostication
The concept that solid tumors can be comprised of both actively cycling and nonc
-
Methods for Studying Spindle Assembly and Chromosome Condensation in Xenopus Egg Extracts
Methods are presented for preparing cytoplasmic extracts from Xenopus laevis egg
-
Receptor Knock-Out and Knock-In Strategies
Accumulating examples have demonstrated that knock-out and knock-in mice of G-pr
-
Pre-embedding Nanogold Silver and Gold Intensification
Pre-embedding nanogold silver and gold intensification methods involve immunorea
-
Purification of Native Bone Marrow Megakaryocytes for Studies of Gene Expression
Megakaryocytes constitute less than 1% of all marrow cells, therefore purificati
