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Ex Vivo Generation of Human Red Blood Cells: A New Advance in Stem Cell Engineering
We describe a technological approach permitting the massive expansion of CD34+ s
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Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton
Ran is a GTPase of the Ras superfamily. Until recently, its primary role was bel
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内体(endosome)
内体是膜包裹的囊泡结构,有初级内体(early endosome)和次级内体(late endosome)之分, 初级内体通常位于细胞质的外侧,次级内体常位于细
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Assessment of Myocardial Angiogenesis and Vascularity in Small Animal Models
Therapies that aim to prevent myocardial tissue from dying or to regenerate new
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Real-Time PCR Quantification of Gene Expression in Embryonic Mouse Tissue
The Gbx family of transcription factors consists of two closely related proteins
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Atomic Force Microscopy for the Characterization of Proteoliposomes
Atomic Force Microscopy (AFM) is a useful tool for the visualization of soft bio
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Nuclear Transplantation and Cloning in Mammals
Nuclear transplantation has been used for many years as an investigative tool wi
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Alkaline Nuclear Dispersion Assays for the Determination of DNA Damage at the Single Cell Level
Over the past three decades the development of methods for visualizing at the ce
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A High-Throughput siRNA Screening Platform to Identify MYC-Synthetic Lethal Genes as Candidate Thera
Targeted therapeutics toward specific genes and pathways represent the future of
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Small-Molecule Collection and High-Throughput Colorimetric Assay to Identify PARP1 Inhibitors
During the last few years, poly(ADP-ribose)polymerase (PARP) proteins became a v
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组成型分泌途径(constitutive secretory pathway)
在这种分泌途径中, 运输小泡持续不断地从高尔基体运送到细胞质膜,并立即进行膜的融合,将分泌小泡中的蛋白质释放到细胞外, 此过程不需要任何信号的触发, 它存在于所
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Detection of NF-B Activity in Skeletal Muscle Cells by Electrophoretic Mobility Shift Analysis
An electrophoretic mobility shift assay (EMSA) is a common and invaluable techni
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Methods for Iodination of Active TGF, TGF Receptor Crosslinking and Immunoprecipitation of TGF-Recep
Radioiodinated transforming growth factors ( TGF-βs) are critical reagents for f
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Analysis of Neuromuscular Junctions: Histology and In Vivo Imaging
The formation of new synapses within neuronal circuits is considered a primary m
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Feeder-Independent Maintenance of Human Embryonic Stem Cells and Directed Differentiation into Endot
Human embryonic stem cells (hESCs) represent a promising source of cells for mod