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细胞生物基本方法:结缔组织类细胞培养
结缔组织类细胞培养1 )成纤维细胞培养用人或动物胚体为好;动物可用小鼠或鸡胚,去头和内脏,剪成小碎块后,用胰蛋白酶消化法培养;如为人胚,可取皮肤培养。幼儿包皮是
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Treatment of Rat Glioma With Electrochemotherapy
The first attempt to apply electrochemotherapy (ECT) to the brain was reported i
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紫杉醇(taxol)
紫杉醇是红豆杉属植物中的一种复杂的次生代谢产物, 也是目前所了解的惟一一种可以促进微管聚合和稳定已聚合微管的药物。同位素示踪表明, 紫杉醇只结合到聚合的微管上,
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原肌球蛋白(tropomyosin, Tm)
原肌球蛋白是细肌丝中与肌动蛋白的结合蛋白,分子量为235kDa, 长为41nm, 由两条平行的多肽链组成α螺旋构型,每条原肌球蛋白首尾相接形成一条连续的链同肌动
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Proteomics Analysis of Contact-Initiated Eph ReceptorEphrin Signaling
Large-scale biochemical analysis of cell-specific signaling can be interrogated
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Identifying and Counting Protein Modifications Triggered by Nitrosative Stress
Nitric oxide (NO) is a small, labile molecule that plays a key role as an interc
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Measurement and Illustration of Immune Interaction After Stem Cell Transplantation
A variety of stem cells, including embryonic, mesenchymal, and hematopoietic ste
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Antisense Therapeutics: A Promise Waiting to Be Fulfilled
During the past decade, only one antisense-based therapy has received full Food
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Metal Analysis With Capillary Zone Electrophoresis
Capillary electrophoresis has recently attracted considerable attention as a pro
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Culture of Preimplantation Mouse Embryos
The preimplantation mammalian embryo develops as a free living entity within the
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Derivation and Characterization of Thyrocyte-Like Cells From Embryonic Stem Cells In Vitro
The thyroid gland is centrally important in metabolic homeostasis, growth, and d
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Receptor-DNA Interactions: EMSA and Footprinting
Defining the precise promoter DNA sequence motifs where nuclear receptors and ot
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细胞的冻存和复苏
相关专题 一、原理 在不加任何条件下直接冻存细胞时,细胞内和外环境中的水都会形成冰晶,能导致细胞内发生机械损伤、电解质升高、渗透压改变、脱水、PH改变、蛋白
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Virus Vectors for Gene Therapy of the Nervous System
Advances in molecular virology, and in understanding the molecular basis of huma
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Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantation
Dendritic cells (DCs) are uniquely specialized antigen-presenting cells (APC) th