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Conditional Gene Mutagenesis in B-Lineage Cells
Since its first application in mice almost 10 yr ago, the Cre/loxP has become th
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Lentivirus-Mediated Modification of Pluripotent Stem Cells
Relatively safe, HIV-1-based lentiviral vectors have served as an efficient mean
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Immobilization of Photosynthetic Membranes in an Albumin-Glutaraldehyde Crosslinked Matrix
The photosynthetic membranes of chloroplasts are responsible for capturing light
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Cryopreservation of Algae
Eukaryotic microalgae and prokaryotic cyanobacteria (blue-green algae) have trad
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Gene Targeting in Human Pluripotent Stem Cells
Targeted homologous recombination (HR) is an essential tool in stem cell biology
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Analysis of Embryonic Vascular Morphogenesis
The circulatory system is the first organ system to develop in the embryo. It is
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Assay of Protein Kinases and Protein Phosphorylation
Part A. Protein Kinase-Mediated Phosphorylation Events Protein kinases (ATP:prot
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The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Pr
This chapter explores the combination of a nonradioactive in situ hybridization
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The Isolation of Membrane Proteoglycans
The proteoglycans (PGs) are a large and varied family of complex macromolecules
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Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases
Polyamine oxidation is the main catabolic process of polyamines. This process is
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Profiling Stem Cells Using Quantitative PCR Protein Assays
Reprogramming human somatic cells to induced pluripotent stem cells is an import
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Evolution of the Wnt Pathways
Wnt proteins mediate the transduction of at least three major signaling pathways
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Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled with Fluorescent In Situ Hybridization
Hedgehog (Hh) family members are secreted proteins that can act at short and lon
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Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization
In blood vessel development (vasculogenesis) smooth muscle cells (SMCs) are deri
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Methods to Analyze Cellular Necroptosis
Necroptosis is a mechanism of necrotic cell death induced by external stimuli in
