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The Analysis of Intermediate Filament Dynamics Using Transfections and Cell Fusions
The intermediate filament (IF) proteins have been recently found as dynamic stru
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探讨琼脂糖凝胶电泳在分子生物学研究中的发展趋势
1. DNA的分离与鉴定大片段DNA的分离:琼脂糖凝胶电泳特别适用于分离大片段的DNA。它能够区分相差100bp的DNA片段,且制备容易,分离范围广。普通琼
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Primary Human Osteoblast Cultures
Osteoblast cultures can be used to investigate the mechanisms of bone formation,
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细胞工程技术(cell engineering)
细胞工程技术是细胞生物学与遗传学的交叉领域,主要利用细胞生物学的原理和方法,结合工程学的技术手段,按照人们预先的设计,有计划地改变或创造细胞遗传性的技术。包括体
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Silencing of Rho-GDI by RNAi Promotes the Differentiation of Neural Stem Cells
RNA interference (RNAi) technology is one of the main means in the study of stem
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Induction of Cellular Senescence by Oncogenic RAS
Cellular senescence is a state of permanent cell cycle arrest, which has been sh
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Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer
cAMP is a ubiquitous second messenger that controls numerous cellular events inc
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In Vivo Matrigel Migration and Angiogenesis Assay
The search for rapid and reproducible in vivo angiogenesis and antiangiogenesis
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Two-Photon Intravital Multicolour Imaging to Study Metastatic Behaviour of Cancer Cells In Vivo
In the last decade, intravital microscopy on breast tumours in mice at single-ce
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The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and Recovery
The spindle checkpoint is a surveillance mechanism that ensures the fidelity of
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Microinjection into Zebrafish Embryos
Microinjection remains the most popular and effective of the methods to introduc
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Descriptive Statistics
Statistics is defined by the Medical Subject Headings (MeSH) thesaurus as the sc
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Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System
Embryonic stem (ES) cells have the potential to proliferate infinitely in vitro
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核孔复合物(nuclear pore complexs,NPCs)
核被膜上有许多孔, 称为核孔( nuclear pore ),是细胞核膜上沟通核质与胞质的开口, 由内外两层膜的局部融合所形成, 核孔的直径为80~120nm。
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Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry
An important topic in membrane biochemistry is the determination of the sidednes