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Random Mutagenesis of Short Target DNA Sequences via PCR with Degenerate Oligonucleotides
Polymerase chain reaction (PCR) is a powerful technique for the amplification of
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Analysis of Nuclear UracilDNA Glycosylase (nUDG) Turnover During the Cell Cycle
Uracil–DNA glycosylases (UDG/UNG) are enzymes that remove uracil from DNA and in
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Detection of Cell Death in Drosophila
Drosophila is a powerful model system for the identification of cell death genes
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Chromatin Immunoprecipitation for Studying Transcriptional Regulation in Xenopus Oocytes and Tadpole
Understanding the accurate temporal and spatial regulation of gene expression du
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Adult Stem Cells for the Treatment of Neurological Disease
The characteristic CNS responses to injury including increased cell production a
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Long-Circulating, pH-Sensitive Liposomes
A major limiting factor for the wide application of pH-sensitive liposomes is th
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Detection of Nuclear -catenin in Xenopus Embryos
Immunodetection of β-catenin accumulation in the nucleus is the most direct and
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Zymographical Techniques for Detectionof Matrix Metalloproteinases
Matrix metalloproteinases (MMPs) are a family of zinc-dependent proteinases asso
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Aging Studies in Drosophila Melanogaster
Drosophila is a genetically tractable system ideal for investigating the mechani
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The Mitochondria of Cultured Mammalian Cells: I: Analysis by Immunofluorescence Microscopy, Histoche
Mitochondria form a dynamic network in which continuous movement, fusion, and di
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Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies
Transfected cell microarrays, arrays of cells expressing defined cDNAs, are prom
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Use of Divalent Metal Ions Chelated to Agarose Derivatives for Reversible Immobilization of Proteins
Immobilization of a protein through coordinate bonds formed with divalent metal
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Analysis of EGF Receptor Interactions With the Subunit of the Stimulatory GTP Binding Protein of Ade
Besides stimulating the mitogen-activated protein kinase, phospholipase Cγ, and
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Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of
Inhibition of DNA replication in eukaryotic cells was one of the earliest effect
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Analysing the ATP Turnover Cycle of Microtubule Motors
Proteins of the kinesin superfamily share a conserved motor domain, which both h
