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Detection by TUNEL labeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling by Boehringer Mannhei
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Automated Isolation and Processing of Adipose-Derived Stem and Regenerative Cells
The popularity of nonhematopoietic, adult tissue-derived stem and progenitor cel
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Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells
This chapter describes the analysis of long- and short-lived plasma cells on tis
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In Situ Hybridization for Metalloproteinases and Their Inhibitors
The imminent completion of the human and mouse genome projects will reveal all m
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Profiling the Metabolic Signature of Senescence
Aging is a complex process, which involves changes in different cellular functio
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COPⅡ被膜小泡(COPⅡ coated vesicle)
由外被蛋白Ⅱ(coat protein Ⅱ, COP Ⅱ )包裹的小泡。外被蛋白是一个大的复合体，称为外被体(coatomer)。这种类型的小泡介导非选择性运输
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Isolation and Purification of Vertebrate DNAs
Isolation of DNA from surrounding tissues is a crucial initial step in any molec
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Magnetic Resonance Imaging Analysis of Embryos
Analyzing phenotypic effects in embryos produced with directed gene manipulation
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原代培养(primary culture)
原代培养是指直接从机体取下细胞、组织和器官后立即进行培养。因此，较为严格地说是指成功传代之前的培养，此时的细胞保持原有细胞的基本性质，如果是正常细胞，仍然保留二
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Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays
This chapter covers gene expression analysis by microarray to study and characte
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Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic Cells
Successful retroviral gene transfer into hematopoietic cells has been demonstrat
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In Vitro Fabrication of Engineered Human Skin
In vitro fabrication of human epidermal tissues that mimic the biochemical and m
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Using Somatic-Cell Nuclear Transfer to Study Aging
In mammals, a diploid genome following fertilization of haploid cells, an egg, a
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Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal
Regulated secretion is a central issue for the specific function of many cells;
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Immunosubtraction as a Means of Typing Monoclonal and Other Proteins in Serum and Urine
Serum and urine protein electrophoresis are used primarily to screen for the pre
