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Isolation and Purification of Vertebrate DNAs
Isolation of DNA from surrounding tissues is a crucial initial step in any molec
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Magnetic Resonance Imaging Analysis of Embryos
Analyzing phenotypic effects in embryos produced with directed gene manipulation
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原代培养(primary culture)
原代培养是指直接从机体取下细胞、组织和器官后立即进行培养。因此,较为严格地说是指成功传代之前的培养,此时的细胞保持原有细胞的基本性质,如果是正常细胞,仍然保留二
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Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays
This chapter covers gene expression analysis by microarray to study and characte
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Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic Cells
Successful retroviral gene transfer into hematopoietic cells has been demonstrat
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In Vitro Fabrication of Engineered Human Skin
In vitro fabrication of human epidermal tissues that mimic the biochemical and m
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Using Somatic-Cell Nuclear Transfer to Study Aging
In mammals, a diploid genome following fertilization of haploid cells, an egg, a
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Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal
Regulated secretion is a central issue for the specific function of many cells;
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Immunosubtraction as a Means of Typing Monoclonal and Other Proteins in Serum and Urine
Serum and urine protein electrophoresis are used primarily to screen for the pre
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Three-Dimensional Alignment of Schwann Cells Using Hydrolysable Microfiber Scaffolds: Strategies for
Injuries to the peripheral nervous system affect 1 in 1,000 individuals each yea
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Solid Tumor DNA Ploidy Analysis
Flow cytometric DNA content analysis of human neoplasia provides quantitative in
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Pheromone Halo Assay
Pheromone Halo Assay REFERENCE: Hoffman, G., Garrison, T. R., and Dohlman, H. G.
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Assaying Phosphoinositide Phosphatases
The roles of phosphoinositide second messengers as signaling molecules in a vast
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Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells
During culture adaptation, human embryonic stem cells (ESCs) and induced pluripo
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细胞培养――细胞生长和细胞毒性
Articles posted in the Method FroumCell Viability Assay Dye exclusion methodViab