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Short-Term Culture of Human CD34+ Cells for Lentiviral Gene Transfer
Haematopoietic Stem Cells (HSCs) are attractive targets for the gene therapy. Up
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Maintenance of Human Embryonic Stem Cells in Animal Serum- and Feeder Layer-Free Culture Conditions
The availability of human embryonic stem cells (hESCs) reflects their outstandin
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Phage-Displayed Antibodies to Detect Cell Markers
Immunologic reagents such as antibodies can be valuable for identifying and isol
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Grb2蛋白(growth factor receptor-bound protein 2)
Grb2是生长因子受体结合蛋白2,又叫Ash蛋白。该蛋白参与细胞内各种受体激活后的下游调节。它能够直接与激活的表皮生长因子受体磷酸化的酪氨酸结合,参与EGF受体
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核型(karyotype)
核型是指染色体组在有丝分裂中期的表型, 是染色体数目、大小、形态特征的总和。在对染色体进行测量计算的基础上, 进行分组、排队、配对, 并进行形态分析的过程叫核型
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The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic
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Receptor Mutagenesis Strategies for Examination of Structure-Function Relationships
This chapter describes three different strategies of receptor mutagenesis with t
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Composite Electrospun Nanofibers for Influencing Stem Cell Fate
The design of new bioactive materials, provided with “instructive properties” an
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Isolation of Lineage-Restricted Neural Precursors from Cultured ES Cells
Embryonic stem (ES) cells are derived from undifferentiated cells present at the
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Matched Cultures of Keratinocytes and Fibroblasts Derived from Normal and NER-Deficient Mouse Models
The uppermost layer of our skin, the epidermis, is formed largely of keratinocyt
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Affinity Precipitation of Proteins Using Metal Chelates
Metal affinity precipitation has been successfully developed as a simple purific
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Analysing Caspase Activation and Caspase Activity in Apoptotic Cells
Apoptotic cell death is characterised by various morphological and biochemical c
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膜运输蛋白(membrane transport protein)
膜运输蛋白是膜整合蛋白, 或是大的跨膜分子复合物, 功能是参与被动运输(促进扩散)或主动运输(运输泵)。参与促进扩散的膜运输蛋白虽然没有酶活性, 但是具有酶催化
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In Vitro Migration Assays of Neural Stem Cells
We describe three rapid procedures for the in vitro investigation of molecular f
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Collagen Gel Culture of the Human Hematopoietic Progenitors CFU-GM, CFU-E, and BFU-E
The first evidence of clonal growth and differentiation of hematopoietic cells w