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Generation of Functional Insulin-Producing Cells from Human Embryonic Stem Cells In Vitro
Human pancreatic islet transplantation at present is the preferred therapeutic o
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Immobilization in Carrageenans
Immobilization in carrageenans is a method of gel entrapment. It is one of the m
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Analysis of Circadian Leaf Movement Rhythms in Arabidopsis thaliana
Arabidopsis thaliana is the model organism for the study of the higher plant cir
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Stem Cell Sources for Regenerative Medicine
Tissue-resident stem cells or primitive progenitors play an integral role in hom
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Promotion of Duplex and Triplex DNA Formation by Polycation Comb-Type Copolymers
Triplex DNA has attracted considerable interest recently because of its possible
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Isolation and Differentiation of MonocytesMacrophages from Human Blood
The prevalence of fungal infections remains high, and it is associated with sign
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神经细胞粘着分子(neural cell adhesion molecule,N-CAM)
N-CAM是膜糖蛋白,至少以三种形式存在,但都是由同一基因编码。其中两种是跨膜蛋白,第三种共价结合在细胞质膜的外表面。无论是何种存在方式,N-CAM分子都有一部
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Multiplex FISH and Spectral Karyotyping
Multicolor fluorescence in situ hybridization (mFISH) assays are now indispensab
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Purification of Adipose Tissue Mesenchymal Stem Cells and Differentiation Toward Hepatic-Like Cells
There is a great interest in the development of functional hepatocytes in vitro
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Permeability of Endothelial Barrier: Cell Culture and In Vivo Models
The methods for assessment of endothelial barrier permeability are vital tools o
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Investigating the Interactions Between Haemopoietic Stem Cells and Their Niche: Methods for the Anal
Interactions between haemopoietic stem cells (HSC) and their microenvironment se
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Flow Cytometric Analysis of Cell Cycle
Fixation 1) Collect 2 X 106 cells. 2) Pellet cells by spinning at 1,000 rpm, 4C
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Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell
Stem cells are undifferentiated cells that have a remarkable ability to self-ren
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Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura- 2
The early methods for calcium measurement involved microinjection of calcium-sen
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Reconstitution of Nuclear Import in Permeabilized Cells
The trafficking of protein and RNA cargoes between the cytoplasm and the nucleus