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Extraction of Nucleic Acids from Bone
Here, we present methods for extracting DNA and RNA from samples of whole bone t
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Scale-Up of Single CellInoculated Suspension Cultures of Human Embryonic Stem Cells
We have developed a simple yet highly reproducible technique of upscaling single
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Preparation of Chick Striated Muscle Cultures
The maturation of striated muscle in primary cultures closely parallels the form
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Regulator of Epidermal Growth Factor Signaling: Sprouty
Sprouty was first discovered through its downregulatory effect on fibroblast gro
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NK细胞介导的细胞溶解作用的研究
前言自然杀伤细胞(Nature Killer Cells, NK)是骨髓衍化的淋巴细胞,其最初通过巨大的颗粒性状被识别。NK细胞能自发杀灭多种肿瘤细胞,同时还可
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Chemiluminescence Assays to Investigate Membrane Expression and Clathrin-Mediated Endocytosis of KAT
Macroscopic ion channel currents (I ) are a product of the channel open probabil
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Use of Murine Embryonic Stem Cells in Embryotoxicity Assays: The Embryonic Stem Cell Test
The embryonic stem cell test (EST) takes advantage of the potential of murine em
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Isolation and Characterization of Side Population Cells
The protocol for isolation of side population (SP) cells was originally establis
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Mammalian Embryo Culture in a Microfluidic Device
Over the last decade, the use of in vitro production of mammalian embryos and th
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Analyzing the Roles of Rho GTPases in Cancer Cell Migration with a Live Cell Imaging 3D-Morphology-B
Rho GTPases are master regulators of cytoskeleton dynamics and therefore regulat
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Isolation of Intact, Functional Mitochondria From the Model Plant Arabidopsis thaliana
The ability to isolate intact, functional mitochondria from plant tissues is a k
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Quantification of Protein Complexes by Blue Native Electrophoresis
Blue native electrophoresis (BNE) is a long established method for the analysis
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Expression of MMPs andTIMPs in Mammalian Cells
Expression of recombinant matrix metalloproteinases (MMPs) and tissue inhibitors
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萤光素酶载体PGL3转染细胞到底要不要加G418
相关专题 总有一种转染方法适合你问:最近要做萤光素酶载体PGL3转染细胞,PGL3载体上面带有neo的抗性标记,有一个老师和我说要培养基里面要加G418筛选,但
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Characterization of RNAProtein Interactions by Phosphorothioate Footprinting and Its Applications to
Analogs of naturally occurring substances obtained by chemical modifications are