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Isolation of Nucleoli from Ehrlich Ascites Tumor Cells and Dynamics of Nascent RNA within Isolated N
Here we describe a new, rapid method for isolating nucleoli from Ehrlich tumor c
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相变(phase transition)
膜的流动镶嵌模型说明生物膜是一种动态的结构, 具有膜脂的流动性(fluidity)和膜蛋白的运动性(mobility)。膜的流动性主要是由膜的双脂层的状态变化引
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Gene Transfer Techniques in Whole Embryo Cultured Post-implantation Mouse Embryos
Gene transfer techniques such as electroporation and lipofection are powerful sy
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Characterization of Synthetic Gene Delivery Vectors by Infrared Spectroscopy
For many decades, infrared (IR) spectroscopy has been used to characterize the s
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XenopusEgg Extracts as a Model System for Analysis of Microtubule, Actin Filament, and Intermediate
It has long been apparent that the three cytoskeletal systems of eukaryotic cell
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Antibody Production and Immunocytochemical Localization of Amino Acid Transporters
This chapter details the procedural and conceptual issues that underpin the loca
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Spatiotemporal Stimulation of Single Cells Using Flow Photolysis
Quantitative studies of chemotactic signaling require experimental techniques th
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Flow Cytometry of Fibroblast Nuclei for DNA content
Materials P.I. Solution :4 mM Na3Citrate (0.118 g/100 mL) 30 U/mL RNAseI (43 mg/
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In Vitro Assays for Axonal Growth and Targeting
In vitro assays are important tools for investigating how the intricate network
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Combinatorial Chemistry in Steroid Receptor Drug Discovery
Combinatorial chemistry has significantly increased the speed of small molecule
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Three-Dimensional Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem Cells
Stem cells can easily be harvested from adipose tissue in large numbers for use
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Tubulin Basics
I. Useful Values1 mg/ml tubulin = 10 µM (assuming MW of ab-tubulin heterodimer i
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Production and Validation of Recombinant Adeno-Associated Virus for Channelrhodopsin Expression in N
Recent discovery of the light-activated ion channel, channelrhodopsin (ChR), has
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Isolation of Circulating Angiogenic Cells
Endothelial progenitor cells (EPCs) were first identified by Ashara et al. in 19
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Purification of U Small Nuclear Ribonucleoprotein Particles
The spliceosome is the catalytic entity that removes the introns from the primar
