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Analysis of Transformation and Tumorigenicity Using Mouse Embryonic Fibroblast Cells
An important step in cellular transformation and tumorigenesis is immortalizatio
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Clonality Detection of Expanded T-Cell Populations in Patients With Multiple Myeloma
Expanded T-cell clones in the peripheral blood of patients with multiple myeloma
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Overcoming Multidrug Resistance in Cancer: Clinical Studies of P-Glycoprotein Inhibitors
Chemotherapy remains the mainstay in the treatment and management of many cancer
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Overview of Novel Anticancer Drug Targets
There has been an explosion in the number of potential molecular targets that ca
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PCR Analysis of Microsatellite Instability
Microsatellites are simple, tandemly repeated DNA sequences which are abundantly
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MHC 四聚体助力细胞治疗基础研究：CTL 表位筛选
据统计，2015 年，我国癌症新发人数为 429.2 万。死亡人数 281.4 万，平均 5 年生存率 36.9%。肺癌、胃癌、肝癌、食管癌占到总数的 57%。
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Human Recombinant Fab Antibodies with T-Cell Receptor-Like Specificities Generated from Phage Displa
In this chapter we describe efficient procedures for the construction, expressio
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Markers for Anti-cytotoxic T-lymphocyte Antigen 4 (CTLA-4) Therapy in Melanoma
Therapeutic strategies that block Cytotoxic T lymphocyte antigen-4 (CTLA-4) enha
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Measuring Cyclin-Dependent Kinase Activity
The activity of cyclin-dependent kinases (CDKs) lies at the heart of cell cycle
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Transgenic Animal Models
Cancer is a prevalent and poorly understood disease in human populations. It is
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Diagnosis and Monitoring of Chronic Myeloid Leukemia by Qualitative and Quantitative RT-PCR
Real-time quantitative reverse-transcription polymerase chain reaction (RQ-PCR)
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galactosidase assay for cells transfected with pSV- -Galactosidase Vector
Chen-Ming Fan Lab,Carnegie Institute of WashingtonPrepare cell lysate as you wou
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Tailoring Kinetics of Antibodies Using Focused Combinatorial Libraries
Antibodies have been used extensively for diagnostic applications for decades. A
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用LIPO2000转染HepG2细胞的问题
乡乡问：最近本人在做HepG2细胞的转染，转染的是GFP质粒，按照LIPO2000的说明书使用后，发现细胞的死亡很严重，24h时差不多死了一半。而且我在转染前细
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腺病毒能在293中复制吗？
腺病毒可以在293A细胞中进行转录，表达并可进行包装复制出大量高滴度病毒。293T是一种逆转录包装表达细胞，我做过也可进行表达，但还没有具体鉴定表达后的病毒是否
