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Cyanogen Bromide digestion of protein
James Movius,Hahn Lab1.Proteins are TCA precipitated and washed with acetone,the
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Terminal Deoxynucleotidyl Transferase
Terminal Deoxynucleotidyl Transferase (TdT) is an enzyme that catalyzes the repe
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Glycolipid Binding Assay
ProcedureA: Preparation of the cell lysate1.Rinse a 60 mm culture dish of conflu
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Northern Blot
Northern Blot I. Electrophoresisclean gel box with NaOH and/or SDS, 2 hours to o
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James Hardwick's angiotensin assay protocol
This specific procedure was developed to assay the activity of the Lck kinase ex
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LABELING GIZZARD MYOSIN WITH IATR (MHC AND 17KD MLC)
Day 1-2Materials1.0.5 M KCl,10 mM HEPES,pH 7.5,4℃,250 ml.2.0.5 M KCl,50 mM HEPES
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Fluorescein labeling of proteins
This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluores
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Dried droplet
The discovery of this method allowed the application of laser desorption to prot
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DNA-Methyltransferase Assay
This protocol was written by Jean-Pierre Issa, based on Adams et al.* Kam-Wing J
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Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
Materials1.Drop frozen 2x cycled tubulin in a polymerization-promoting buffer.St
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Western Blot of TBP from TBP-GST bacteria
Culture bacteria1. Grow glycerol stock A13 (TBP-GST in BL21) in 10ml LB and 10μl
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RNA甲醛琼脂糖凝胶分离的Northern杂交
Northern Blotting技术专题Abstract: This is the standard and complete Northern blotti
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Western的几点经验
1.用脱脂奶粉封闭的效果不一定比用BSA差,不过我们试过几种奶粉,有一些奶粉不太适合.推荐使用的奶粉:雀巢高钙奶粉,多力精低脂奶粉.一般国产的奶粉的颗粒比较大,
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Carbonate Solution For Western Blot
CARBONATE WESTERN TRANSFER SOLUTIONSDunn, Anal. Biochem. 1986: 1571.5L1.0L10mM N
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LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,1 mM PIPES,pH 6.8 at 4℃,250 ml for
