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General PCR methods
Polymerase Chain Reaction (PCR)(adapted from Bruce A. Roe, Department of Chemist
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real-time PCR-Molecular Biology
hi,I am going to do real-time PCR using Smart Cycler, but I have neverdone this
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Long-PCR Reagents and Guidelines
Long-PCR Reagents and Guidelinesfrom George Church as Modified from Cheng et al.
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Isolation of Retroelement from Plant Genomic DNA
The amplification of DNA fragments using the polymerase chain reaction (33) is
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PCR基本实验方法（三）
"Hot Start" PCR: In certain circumstances one wishes to avoid mixing p
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Polymerase Chain Reaction
Materials:Taq (or other) PCR enzyme (5 Units/ul) PCR enzyme reaction buffer
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PCR principles and practices
Important parameters in the PCR:1.Template DNA quantity (complexity determines n
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PCR基本实验方法（四）
Labelling PCR Products with DigoxigeninPCR products may be very conveniently lab
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Primer4.10中文使用说明
Primer4.10中文使用说明Primer Premier4.0是由加拿大的Premier公司开发的专业用于PCR或测序引物以及杂交探针的设计，评估的软件，和
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Reaction volume
Q: Does the PCR reaction volume (negatively) influence the outcome?A: No, especi
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CORE SAMPLE PCR
A method to re-PCR unique bands from products of mixed sizeINTRODUCTIONThe produ
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contamination in real-time pcr negative control-Real-Tim
hi there,i am noew constantly getting a late peak (30+ cycles) in the negative c
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Small amounts of RNA for qRT-PCR-Real-Time PCR
I have a quick question. In our lab we have had a great deal of sucess using qRT
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Polymerase Chain Reaction--热启动PCR
This procedure is a "hot start" PCR cycle for use with large primers.
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Single tube confirmation PCR protoco
The following colony PCR protocol has been designed to be performed in individua
