real-time PCR-Molecular Biology

hi,I am going to do real-time PCR using Smart Cycler, but I have neverdone this experiment, I do not know how to design primers,such as thelength of primers,the size of PCR product and the annealing temperatureet al.If you happen to know the knowledge about it, would you like tosharing with me?Thanks a lot!

-geness-

Here you can find SmartCycler specific information on primer design for real-time PCR. Additional guidelines can be found here If you are going to use SYBR Green I dye, primer design is similar to regular RT-PCR. However, since SYBR Green I dye will detect all double-stranded DNA, including non-specific reaction products, optimization of the reaction conditions for each primer set are very important. SAGE

-sage-

Thank you for your kindly reply ! Best wishes to you!

-geness-

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