Fluorescence Recovery After Photobleaching
This chapter describes the use of microscope-based fluorescence recovery after p
This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as mobile fraction and half-time of recovery.
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Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the Raclure Method
Mesenchymal stem cells also called mesenchymal stromal cells...
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Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dyna
Mitotic spindle microtubules pull chromosomes toward each po...