植物信号转导中的MAPK级联途径

相关专题 MAPK信号通路专题

MAPK diverse_plants：Our current studies focus on five MAPK cascades, including those involved in osmotic stress responses, Flg22 peptide-mediated defense responses, the ethylene pathway, and auxin and H2O2 signaling. The overall strategy involves the identification and cloning of the complete set of Arabidopsis genes encoding MAPKs, MAPKKs, MAPKKKs, and relevant protein phosphatases. Transient expression of epitope-tagged MAPKs, MAPKKs, and MAPKKKs will be used to determine which MAPK cascades respond to particular abiotic or biotic stress or hormone signals in Arabidopsis and maize protoplasts. Constitutively active and dominant negative forms of these MAPKKKs and MAPKKs will be used to identify specific reporter genes associated with particular signaling pathways. DNA array technology will be used to analyze MAPK-related gene expression patterns, and to reveal new downstream targets and crosstalk between specific MAPK cascades. Identification of Arabidopsis knockout mutants corresponding to key MAPK-related regulatory genes will be initiated. Transgenic Arabidopsis, maize and soybean plants that overexpress particular engineered MAPKKK and MAPKK genes will be generated. The transgenic plants will be tested for the exhibition of agronomically useful traits.