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Measurement of DNA Damage in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assa

2025-03-13 细胞技术 加入收藏
The Single Cell Gel Electrophoresis (Comet) assay is a simple, versatile and sen

The Single Cell Gel Electrophoresis (Comet) assay is a simple, versatile and sensitive method for measuring DNA damage in individual cells, allowing the determination of heterogeneity of response within a cell population. The basic alkaline technique described is for the determination of DNA strand break damage and its repair at a single cell level. Specific modifications to the method use a lower pH (‘neutral’ assay), or allow the measurement of DNA interstrand cross-links. It can be further adapted to, for example, study specific DNA repair mechanisms, be combined with fluorescent in situ hybridisation, or incorporate lesion specific enzymes.

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