Xenografting and Harvesting Human Ductal Pancreatic Adenocarcinomas for DNA Analysis
Xenotransplantation (xenografting) of primary cancers or cancer cell lines into
Xenotransplantation (xenografting) of primary cancers or cancer cell lines into immunodeficient mice is a commonly used technique to assess tumor growth in response to a variety of experimental agents. When primary pancreatic cancers are xenografted, cancer cells proliferate in the mouse, but the human stroma does not. This growth pattern enables facile genetic analysis of cancer genetics profiles without the contamination of admixed stromal cells typical of primary cancers.
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Isolation of RNA From Tumor Samples: Single-Step Guanidinium Acid-Phenol Method
The guanidinium acid-phenol method of RNA extraction is rela...
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Determination of Cyclin D1 Expression by Quantitative Real-Time, Reverse-Transcriptase Polymerase Ch
Disorders of the cell cycle regulatory machinery play a key ...