LIPID EXTRACTION

PROCEDURE

1. Resuspend tissue/extract in 5 volumes of 1:1 chloroform/methanol by vortexing (to homogeneity or as close as possible)

2. Add the same volume of a 1M KCl, 0.2M Phosphoric acid solution and vortex again

3. Centrifuge sample at 10,000 xg for 2 minutes

4. Collect the bottom phase (contains lipids)