Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 生物化学

生物化学

Detection of Phosphatidylinositol-4-Phosphate 5-Kinase Activity Using Thin-Layer Chromatography

2025-03-25 生物化学 加入收藏
Phosphatidylinositol-4-phosphate 5-kinases (PIP5Ks; EC 2.7.1.68) synthesize phos

Phosphatidylinositol-4-phosphate 5-kinases (PIP5Ks; EC 2.7.1.68) synthesize phosphatidylinositol 4,5-bisphosphate (PI 4,5-P2 ) from phosphatidylinositol 4-phosphate (PI 4-P). The hydrolysis of PI 4,5-P2 by phosphoinositide-specific phospholipase C generates the second messengers 1,2-diacylglycerol, which activates several protein kinase C isoforms, and inositol 1,4,5-trisphosphate, which stimulates the release of calcium from intracellular stores (1 ). PI 4,5-P2 can also be phosphorylated by the PI 3-kinase, generating phosphatidylinositol 3,4,5-trisphosphate (PI 3,4,5-P3 ), a second messenger of generally unknown function; however, some isoforms of protein kinase C may be targets (2 ) as well as proteins that contain pleckstrin homology (PH) domains (3 ). Furthermore, PI 4,5-P2 regulates multiple enzymes and several actin-binding proteins (4 ), is bound by PH domains found in some signaling proteins (5 ), and appears to play a role in the secretory vesicle cycle (6 ,7 ).

文章底部广告位

文章评论

加载中~