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Assay and Purification of Calmodulin-Dependent Protein Kinase

2025-03-25 生物化学 加入收藏
Posttranslation covalent modification of proteins by phosphorylation represents

Posttranslation covalent modification of proteins by phosphorylation represents an important mechanism in the control of many cellular functions (1 ,2 ). Protein kinases catalyze the transfer of the γ-phosphoryl group of ATP to an acceptor protein substrate. The activity of the enzyme is determined by the transfer of 32 P from [γ-32 P] ATP to protein substrate

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