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Purification and cloning of DNA fragments fractionated on agarose gels

2025-03-25 生物化学 3 0 加入收藏
Purification of DNA fragments from acrylamide or agarose gels is a commonly used

Purification of DNA fragments from acrylamide or agarose gels is a commonly used technique in the molecular biology laboratory. This article describes a rapid, efficient, and inexpensive method of purifying DNA fractions from an agarose gel. The purified DNA is suitable for use in a wide range of applications including ligation using DNA ligase. The procedure uses standard high-melting-temperature agarose and normal TBE electrophoresis buffer. In addition, the protocol does not involve the use of highly toxic organic solvents such as phenol.

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