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Quantitation of Ribozyme Target Abundance by QCPCR

2025-03-27 生物化学 加入收藏
Ribozymes show promise as therapeutic agents for the downregulation of specific

Ribozymes show promise as therapeutic agents for the downregulation of specific target RNAs inside cells. To demonstrate the efficacy of a given ribozyme treatment, one needs to show that the treatment ameliorates the disease condition relative to a control treatment. As part of that demonstration, it is useful to show that treatment with ribozyme also results in a lowered abundance of the target RNA (see Note 1 ). This chapter describes the application of the quantitative, competitive polymerase chain reaction (QCPCR) technique for evaluating the abundance of RNAs targeted for ribozyme-mediated destruction.

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