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Nitrocellulose-Based Immunoaffinity Chromatography

2025-03-28 生物化学 加入收藏
The first step in the affinity purification of antibodies is usually the immobil

The first step in the affinity purification of antibodies is usually the immobilization of the corresponding antigen to a solid-phase matrix. Generally, this immobilization is done using some chemically reactive gel material such as cyanogen-bromide activated Sepharose. In fact, for large-scale preparative purifications this is an appropriate method to generate columns with adequate binding capacities and flow properties. However, for many purposes particu-late nitrocellulose (NC) is a promising alternative material, which is characterized by several advantageous properties, some of which are outlined as follows.

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