Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish
This chapter describes a method for generation of the high-titer pseudotyped Mol
This chapter describes a method for generation of the high-titer pseudotyped Moloney murine leukemia virus (MLV) that efficiently infects zebrafish embryos (i.e., more than 25 retroviral copies per cell). Injection techniques are also described for production of the retrovirus-infected mosaic “founder” fish. We describe a quantitative PCR (qPCR)-based assay as a quick way to assess the infectivity after each round of viral production and injection. Most of the required equipment is commercially available and commonly present in most research laboratories.
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Protein Fingerprinting of Staphylococcus aureus by Capillary Electrophoresis with On-Capillary Deriv
This chapter describes a complete procedure for obtaining pr...
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Preparation of Oriented, Fully Hydrated Lipid Samples for Structure Determination Using X-Ray Scatte
This chapter describes a method of sample preparation called...