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Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish

2025-03-29 生物化学 加入收藏
This chapter describes a method for generation of the high-titer pseudotyped Mol

This chapter describes a method for generation of the high-titer pseudotyped Moloney murine leukemia virus (MLV) that efficiently infects zebrafish embryos (i.e., more than 25 retroviral copies per cell). Injection techniques are also described for production of the retrovirus-infected mosaic “founder” fish. We describe a quantitative PCR (qPCR)-based assay as a quick way to assess the infectivity after each round of viral production and injection. Most of the required equipment is commercially available and commonly present in most research laboratories.

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