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Calibration of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Peptide Mass Fingerprintin

2025-03-29 生物化学 加入收藏
This chapter describes a number of aspects important for calibration of matrix-a

This chapter describes a number of aspects important for calibration of matrix-assisted laser desorption/ionization time-of-flight spectra prior to peptide mass fingerprinting searches. Both multipoint internal calibration and mass defect-based calibration is illustrated. The chapter describes how potential internal calibrants, like tryptic autodigest peptides and keratin-related peptides, can be identified and used for high-precision calibration. Furthermore, the construction of project/user-specific lists of potential calibrants is illustrated.

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