Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes
This chapter describes a nonradioactive method for the localization of mRNA in w
This chapter describes a nonradioactive method for the localization of mRNA in whole mouse embryos. It employs riboprobes labeled with digoxigenin, a steroid-like moiety not found in animal tissue. Digoxigenin-containing probe is visualized with a conjugate of antidigoxigenin Fab and alkaline phosphatase and colorimetric staining. The results are visualized in three dimensions, hence subtle patterns can be visualized without laborious sectioning.
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Preparation of Oriented, Fully Hydrated Lipid Samples for Structure Determination Using X-Ray Scatte
This chapter describes a method of sample preparation called...
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Calibration of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Peptide Mass Fingerprintin
This chapter describes a number of aspects important for cal...