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Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes

2025-03-29 生物化学 加入收藏
This chapter describes a nonradioactive method for the localization of mRNA in w

This chapter describes a nonradioactive method for the localization of mRNA in whole mouse embryos. It employs riboprobes labeled with digoxigenin, a steroid-like moiety not found in animal tissue. Digoxigenin-containing probe is visualized with a conjugate of antidigoxigenin Fab and alkaline phosphatase and colorimetric staining. The results are visualized in three dimensions, hence subtle patterns can be visualized without laborious sectioning.

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