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Eukaryotic Cell Labeling and Preparation for 2-D

2025-03-31 生物化学 加入收藏
Two-dimensional polyacrylamide gel electrophoresis (2-DE) provides the ability t

Two-dimensional polyacrylamide gel electrophoresis (2-DE) provides the ability to resolve and quantify thousands of proteins from an unfractionated eukaryotic cell lysate. The use of 2-DE in studying eukaryotic cells has run the gambit from ozone-stressed Norway spruce needles (1 ), mapping proteins in the differentiation of mouse endoderm, mesoderm, and ectoderm (2 ) to the study of apoptosis (3 ).

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