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Bacterial Genome Mapping by Two-Dimensional Pulsed-Field Gel Electrophoresis (2D-PFGE)

2025-03-31 生物化学 加入收藏
Two-dimensional pulsed-field gel electrophoresis (2D-PFGE) is a powerful PFGE te

Two-dimensional pulsed-field gel electrophoresis (2D-PFGE) is a powerful PFGE technique for the restriction mapping of bacterial genomic DNA. The method consists of two sequential steps of restriction endonuclease digestion and separation of the fragments by PFGE:

1. Step A, in which partially or completely digested bacterial DNA is separated by PFGE in the first dimension; and
2. Step B, in which a gel slice containing the separated DNA of the first dimension is cut out from the first gel, redigested with the same or a different restriction enzyme, and separated by PFGE in the second dimension, i.e., perpendicular to the first dimension.


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