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Incorporation of Unnatural Non--Amino Acids into the N-Terminus of Proteins in a Cell-Free Translati

2025-03-31 生物化学 加入收藏
Unnatural amino acid mutagenesis allows us to introduce unnatural α-amino acids

Unnatural amino acid mutagenesis allows us to introduce unnatural α-amino acids into internal positions of proteins in response to expanded codons such as amber and four-base codons. To improve the unnatural amino acid mutagenesis, the incorporation of unnatural α-amino acids and non-α-amino acids into the N-terminus of proteins has been achieved using expanded initiation codons. Here, we describe the method for the incorporation of fluorescent-labeled non-α-amino acids into the N-terminus of proteins in a cell-free translation system.

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