In Vitro O-Antigen Ligase Assay
WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at th
WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the proximal end of the undecaprenyl-diphosphate (Und-PP)-O-antigen with a terminal sugar of the lipid A-core oligosaccharide (OS). This is a critical step in lipopolysaccharide synthesis. We describe here an assay to perform the ligation reaction in vitro utilizing native substrates.
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Whole-Cell Patch-Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterol
Voltage-sensitive calcium channels (VSCC) are vital to the n...
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Quantification of Proteins and Metabolites by Mass Spectrometry Without Isotopic Labeling
We demonstrate the quantification capability and robustness ...
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